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well v bottom plate corning  (Greiner Bio)


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    Greiner Bio well v bottom plate corning
    Well V Bottom Plate Corning, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 96/100, based on 430 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/well+v+bottom+plate+corning/Microplate+96+Well+Pp+V-Bottom/pm40060890-519-82-99
    Average 96 stars, based on 430 article reviews
    well v bottom plate corning - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: JNJ-78306358, a first-in-class bispecific T cell engaging antibody targeting CD3 and HLA-G.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Pierce 660nm Protein Assay Reagent ThermoFisher Scientific 22660 Pluronic acid, 10% F-68 Gibco 24040-032 Protease Inhibitor Mini Tablets ThermoFisher Scientific A32955 Protein block, serum free Agilent Dako X0909 Puromycin Gibco A1113803 QuantumTM Simply Cellular beads Bangs Lab anti-mouse, #815B; anti-human, #816A Reaction buffer (10X) Roche 950-300 Read buffer 4x Meso Scale Discovery R92TC-3 RIPA Buffer ThermoFisher Scientific 89901 RPMI 1640 Gibco 61870-036 RPMI 1640 Sigma R0883 RPMI 1640 without phenol red Gibco 32404014 Sodium pyruvate Sigma S8636 Sodium pyruvate Gibco 11360070 Sodium selenite Sigma S5261 Stain buffer (with FBS or BSA) BD Pharmingen 554657 or 554656 Surfactant Meso Scale Discovery R92TC-2 Sytox Blue ThermoFisher Scientific S11348 Sytox Green Dead Cell Stain ThermoFisher Scientific S34860 TBST (TBS with 0.1% Tween20) Bio-Rad 170-6531 Total protein assay module ProteinSimple DM-TP-01 T-PER buffer Pierce 78510 Transfer Buffer Novex NP0006-1 V-PLEX and V-PLEX Plus Proinflammatory Panel 1 (human) Kit Meso Scale Discovery K15049D Wash buffer (1XDPBS, 0.05% Tween-20) Janssen R&D N/A XCell II Blot Module ThermoFisher Scientific EI9051 Xylene VWR 28975-325 Critical commercial assays Anti-HQ-HRP detection kit Roche 701793601 BCA Protein Assay Kit ThermoFisher Scientific 23227 Bond Polymer Refine Detection Kit Leica Biosystems DS9800 DISCOVERY ChromoMap DAB kit Roche 760-159 MACS Human Tumor/Xenograft Dissociation kit Miltenyi Biotec 130-095-929 MACS Mouse Tumor Dissociation kit Miltenyi Biotec 130-096-730 Mouse cell Depletion Kit Miltenyi Biotec 130-104-694 OmniMap DAB anti-Rb Detection Kit Roche 760-149 sHLA-G ELISA kit ExBio RD194070100R T Cell Activation / Expansion Kit, human Miltenyi Biotec 130-091-441 Trypan blue 0.4% Gibco 15250 TrypLE Gibco 12563-029 TrypLE Express Gibco 12605036 TrypLE select Invitrogen 12563-011 TrypLE TM Select Enzyme (13), no phenol red Thermo Fisher Scientific 12563011 UltraPure LMP agarose Invitrogen 16520-050 IL-2 Miltenyi Biotec 130-097-743 Insulin Gibco 12585-014 (Continued on next page) e3 iScience 28, 111876, March 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human IFN-y R&D Systems 285-IF-100 Recombinant human IL-6 R&D Systems 206-IL-200 Recombinant human TNF-a R&D Systems 210-TA-100 Transferrin Sigma T8158 Biotinylated sHLA-G (MHGW8.ECO.PP.DB.002) Janssen R&D NA 0.5 mL Polypropylene assay blocks Bio-one 786261 1.2 mL Deepwell 96-Well plate Assay, polypropylene ThermoFisher Scientific, Abgene AB1127 10 mL Syringe BD Biosciences 301304 15 mL Conical tube Falcon 352097 6-well Clear flat bottom cell culture plate Corning 353046 70-mm Strainer Falcon 352350 96-Well Polypropylene plate Greiner 650201 or 786261 96-Well V bottom plate Corning 3894 96-Well Microplate, Polypropylene, U – Bottom, Chimney Style, Clear, No Lid Greiner Bio-One 650261 96-Well E-Plates ACEA Biosciences/Agilent 5232368001 AbgeneTM 96-Well Polypropylene DeepWell Storage Plates ThermoFisher Scientific AB1127 Albumin standard Pierce 23209 BD Microtainer tube (serum separator) VWR VT365967 Breath-easy membrane Sigma Z380059 Cell Strainer, 70 mm, white Falcon 352350 Deep well storage plates, 96-well, 2.2 ml, Mark II VWR, Abgene 732-4910 Falcon 50 ml conical tubes ThermoFisher Scientific 12716688 Falcon 96-well U-bottom plate Corning 353077 Falcon Centrifuge Tubes, Polypropylene, Sterile, 50 mL Conical Tube Corning 352070 Flat bottom tissue culture plate 96-well Corning 3598 GentleMACS C-Tubes Miltenyi 130-093-237 iBlot Gel Transfer Stacks, PVDF regular Invitrogen IB401001 Immun-Blot PVDF membrane Bio-Rad 162-0177/ 162-0174 Liquid coverslip Roche 650-10 Low attachment 96-well U-bottom plate Costar 7007 Lysing Matrix D tube MP Biomedicals 6913-100 MSD 10-plex plates Meso Scale Discovery K15235N MSD 96-Well Streptavidin Gold Plate Meso Scale Discovery L15SA-1 MSD Small Spot Plate Meso Scale Discovery L45SA-1 Nunc seal Nunc 236366 Sterile plate covers/ Lid Universal Clear Sterile PS ThermoFisher Scientific 250002 Sterile U-bottom 96-wells plates for flow Falcon 351177 TC 5-layer flask, 875 mm2 Falcon 353144 V-bottom 96-well plate Nunc NUNC249952 V-bottom polypropylene 96 well plates Greiner 65261 VWR Micro slides, Superfrost Plus VWR 48311-703 Experimental models: Cell lines .. RERF-LD-Ad1 Radiation Effects Research Foundation JCRB1020 BICR6 Sigma-Aldrich 5070501 (Continued on next page) iScience 28, 111876, March 21, 2025 e4

    Cell Culture:

    Article Title: JNJ-78306358, a first-in-class bispecific T cell engaging antibody targeting CD3 and HLA-G.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Pierce 660nm Protein Assay Reagent ThermoFisher Scientific 22660 Pluronic acid, 10% F-68 Gibco 24040-032 Protease Inhibitor Mini Tablets ThermoFisher Scientific A32955 Protein block, serum free Agilent Dako X0909 Puromycin Gibco A1113803 QuantumTM Simply Cellular beads Bangs Lab anti-mouse, #815B; anti-human, #816A Reaction buffer (10X) Roche 950-300 Read buffer 4x Meso Scale Discovery R92TC-3 RIPA Buffer ThermoFisher Scientific 89901 RPMI 1640 Gibco 61870-036 RPMI 1640 Sigma R0883 RPMI 1640 without phenol red Gibco 32404014 Sodium pyruvate Sigma S8636 Sodium pyruvate Gibco 11360070 Sodium selenite Sigma S5261 Stain buffer (with FBS or BSA) BD Pharmingen 554657 or 554656 Surfactant Meso Scale Discovery R92TC-2 Sytox Blue ThermoFisher Scientific S11348 Sytox Green Dead Cell Stain ThermoFisher Scientific S34860 TBST (TBS with 0.1% Tween20) Bio-Rad 170-6531 Total protein assay module ProteinSimple DM-TP-01 T-PER buffer Pierce 78510 Transfer Buffer Novex NP0006-1 V-PLEX and V-PLEX Plus Proinflammatory Panel 1 (human) Kit Meso Scale Discovery K15049D Wash buffer (1XDPBS, 0.05% Tween-20) Janssen R&D N/A XCell II Blot Module ThermoFisher Scientific EI9051 Xylene VWR 28975-325 Critical commercial assays Anti-HQ-HRP detection kit Roche 701793601 BCA Protein Assay Kit ThermoFisher Scientific 23227 Bond Polymer Refine Detection Kit Leica Biosystems DS9800 DISCOVERY ChromoMap DAB kit Roche 760-159 MACS Human Tumor/Xenograft Dissociation kit Miltenyi Biotec 130-095-929 MACS Mouse Tumor Dissociation kit Miltenyi Biotec 130-096-730 Mouse cell Depletion Kit Miltenyi Biotec 130-104-694 OmniMap DAB anti-Rb Detection Kit Roche 760-149 sHLA-G ELISA kit ExBio RD194070100R T Cell Activation / Expansion Kit, human Miltenyi Biotec 130-091-441 Trypan blue 0.4% Gibco 15250 TrypLE Gibco 12563-029 TrypLE Express Gibco 12605036 TrypLE select Invitrogen 12563-011 TrypLE TM Select Enzyme (13), no phenol red Thermo Fisher Scientific 12563011 UltraPure LMP agarose Invitrogen 16520-050 IL-2 Miltenyi Biotec 130-097-743 Insulin Gibco 12585-014 (Continued on next page) e3 iScience 28, 111876, March 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human IFN-y R&D Systems 285-IF-100 Recombinant human IL-6 R&D Systems 206-IL-200 Recombinant human TNF-a R&D Systems 210-TA-100 Transferrin Sigma T8158 Biotinylated sHLA-G (MHGW8.ECO.PP.DB.002) Janssen R&D NA 0.5 mL Polypropylene assay blocks Bio-one 786261 1.2 mL Deepwell 96-Well plate Assay, polypropylene ThermoFisher Scientific, Abgene AB1127 10 mL Syringe BD Biosciences 301304 15 mL Conical tube Falcon 352097 6-well Clear flat bottom cell culture plate Corning 353046 70-mm Strainer Falcon 352350 96-Well Polypropylene plate Greiner 650201 or 786261 96-Well V bottom plate Corning 3894 96-Well Microplate, Polypropylene, U – Bottom, Chimney Style, Clear, No Lid Greiner Bio-One 650261 96-Well E-Plates ACEA Biosciences/Agilent 5232368001 AbgeneTM 96-Well Polypropylene DeepWell Storage Plates ThermoFisher Scientific AB1127 Albumin standard Pierce 23209 BD Microtainer tube (serum separator) VWR VT365967 Breath-easy membrane Sigma Z380059 Cell Strainer, 70 mm, white Falcon 352350 Deep well storage plates, 96-well, 2.2 ml, Mark II VWR, Abgene 732-4910 Falcon 50 ml conical tubes ThermoFisher Scientific 12716688 Falcon 96-well U-bottom plate Corning 353077 Falcon Centrifuge Tubes, Polypropylene, Sterile, 50 mL Conical Tube Corning 352070 Flat bottom tissue culture plate 96-well Corning 3598 GentleMACS C-Tubes Miltenyi 130-093-237 iBlot Gel Transfer Stacks, PVDF regular Invitrogen IB401001 Immun-Blot PVDF membrane Bio-Rad 162-0177/ 162-0174 Liquid coverslip Roche 650-10 Low attachment 96-well U-bottom plate Costar 7007 Lysing Matrix D tube MP Biomedicals 6913-100 MSD 10-plex plates Meso Scale Discovery K15235N MSD 96-Well Streptavidin Gold Plate Meso Scale Discovery L15SA-1 MSD Small Spot Plate Meso Scale Discovery L45SA-1 Nunc seal Nunc 236366 Sterile plate covers/ Lid Universal Clear Sterile PS ThermoFisher Scientific 250002 Sterile U-bottom 96-wells plates for flow Falcon 351177 TC 5-layer flask, 875 mm2 Falcon 353144 V-bottom 96-well plate Nunc NUNC249952 V-bottom polypropylene 96 well plates Greiner 65261 VWR Micro slides, Superfrost Plus VWR 48311-703 Experimental models: Cell lines .. RERF-LD-Ad1 Radiation Effects Research Foundation JCRB1020 BICR6 Sigma-Aldrich 5070501 (Continued on next page) iScience 28, 111876, March 21, 2025 e4

    Membrane:

    Article Title: JNJ-78306358, a first-in-class bispecific T cell engaging antibody targeting CD3 and HLA-G.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Pierce 660nm Protein Assay Reagent ThermoFisher Scientific 22660 Pluronic acid, 10% F-68 Gibco 24040-032 Protease Inhibitor Mini Tablets ThermoFisher Scientific A32955 Protein block, serum free Agilent Dako X0909 Puromycin Gibco A1113803 QuantumTM Simply Cellular beads Bangs Lab anti-mouse, #815B; anti-human, #816A Reaction buffer (10X) Roche 950-300 Read buffer 4x Meso Scale Discovery R92TC-3 RIPA Buffer ThermoFisher Scientific 89901 RPMI 1640 Gibco 61870-036 RPMI 1640 Sigma R0883 RPMI 1640 without phenol red Gibco 32404014 Sodium pyruvate Sigma S8636 Sodium pyruvate Gibco 11360070 Sodium selenite Sigma S5261 Stain buffer (with FBS or BSA) BD Pharmingen 554657 or 554656 Surfactant Meso Scale Discovery R92TC-2 Sytox Blue ThermoFisher Scientific S11348 Sytox Green Dead Cell Stain ThermoFisher Scientific S34860 TBST (TBS with 0.1% Tween20) Bio-Rad 170-6531 Total protein assay module ProteinSimple DM-TP-01 T-PER buffer Pierce 78510 Transfer Buffer Novex NP0006-1 V-PLEX and V-PLEX Plus Proinflammatory Panel 1 (human) Kit Meso Scale Discovery K15049D Wash buffer (1XDPBS, 0.05% Tween-20) Janssen R&D N/A XCell II Blot Module ThermoFisher Scientific EI9051 Xylene VWR 28975-325 Critical commercial assays Anti-HQ-HRP detection kit Roche 701793601 BCA Protein Assay Kit ThermoFisher Scientific 23227 Bond Polymer Refine Detection Kit Leica Biosystems DS9800 DISCOVERY ChromoMap DAB kit Roche 760-159 MACS Human Tumor/Xenograft Dissociation kit Miltenyi Biotec 130-095-929 MACS Mouse Tumor Dissociation kit Miltenyi Biotec 130-096-730 Mouse cell Depletion Kit Miltenyi Biotec 130-104-694 OmniMap DAB anti-Rb Detection Kit Roche 760-149 sHLA-G ELISA kit ExBio RD194070100R T Cell Activation / Expansion Kit, human Miltenyi Biotec 130-091-441 Trypan blue 0.4% Gibco 15250 TrypLE Gibco 12563-029 TrypLE Express Gibco 12605036 TrypLE select Invitrogen 12563-011 TrypLE TM Select Enzyme (13), no phenol red Thermo Fisher Scientific 12563011 UltraPure LMP agarose Invitrogen 16520-050 IL-2 Miltenyi Biotec 130-097-743 Insulin Gibco 12585-014 (Continued on next page) e3 iScience 28, 111876, March 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human IFN-y R&D Systems 285-IF-100 Recombinant human IL-6 R&D Systems 206-IL-200 Recombinant human TNF-a R&D Systems 210-TA-100 Transferrin Sigma T8158 Biotinylated sHLA-G (MHGW8.ECO.PP.DB.002) Janssen R&D NA 0.5 mL Polypropylene assay blocks Bio-one 786261 1.2 mL Deepwell 96-Well plate Assay, polypropylene ThermoFisher Scientific, Abgene AB1127 10 mL Syringe BD Biosciences 301304 15 mL Conical tube Falcon 352097 6-well Clear flat bottom cell culture plate Corning 353046 70-mm Strainer Falcon 352350 96-Well Polypropylene plate Greiner 650201 or 786261 96-Well V bottom plate Corning 3894 96-Well Microplate, Polypropylene, U – Bottom, Chimney Style, Clear, No Lid Greiner Bio-One 650261 96-Well E-Plates ACEA Biosciences/Agilent 5232368001 AbgeneTM 96-Well Polypropylene DeepWell Storage Plates ThermoFisher Scientific AB1127 Albumin standard Pierce 23209 BD Microtainer tube (serum separator) VWR VT365967 Breath-easy membrane Sigma Z380059 Cell Strainer, 70 mm, white Falcon 352350 Deep well storage plates, 96-well, 2.2 ml, Mark II VWR, Abgene 732-4910 Falcon 50 ml conical tubes ThermoFisher Scientific 12716688 Falcon 96-well U-bottom plate Corning 353077 Falcon Centrifuge Tubes, Polypropylene, Sterile, 50 mL Conical Tube Corning 352070 Flat bottom tissue culture plate 96-well Corning 3598 GentleMACS C-Tubes Miltenyi 130-093-237 iBlot Gel Transfer Stacks, PVDF regular Invitrogen IB401001 Immun-Blot PVDF membrane Bio-Rad 162-0177/ 162-0174 Liquid coverslip Roche 650-10 Low attachment 96-well U-bottom plate Costar 7007 Lysing Matrix D tube MP Biomedicals 6913-100 MSD 10-plex plates Meso Scale Discovery K15235N MSD 96-Well Streptavidin Gold Plate Meso Scale Discovery L15SA-1 MSD Small Spot Plate Meso Scale Discovery L45SA-1 Nunc seal Nunc 236366 Sterile plate covers/ Lid Universal Clear Sterile PS ThermoFisher Scientific 250002 Sterile U-bottom 96-wells plates for flow Falcon 351177 TC 5-layer flask, 875 mm2 Falcon 353144 V-bottom 96-well plate Nunc NUNC249952 V-bottom polypropylene 96 well plates Greiner 65261 VWR Micro slides, Superfrost Plus VWR 48311-703 Experimental models: Cell lines .. RERF-LD-Ad1 Radiation Effects Research Foundation JCRB1020 BICR6 Sigma-Aldrich 5070501 (Continued on next page) iScience 28, 111876, March 21, 2025 e4

    Sterility:

    Article Title: JNJ-78306358, a first-in-class bispecific T cell engaging antibody targeting CD3 and HLA-G.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Pierce 660nm Protein Assay Reagent ThermoFisher Scientific 22660 Pluronic acid, 10% F-68 Gibco 24040-032 Protease Inhibitor Mini Tablets ThermoFisher Scientific A32955 Protein block, serum free Agilent Dako X0909 Puromycin Gibco A1113803 QuantumTM Simply Cellular beads Bangs Lab anti-mouse, #815B; anti-human, #816A Reaction buffer (10X) Roche 950-300 Read buffer 4x Meso Scale Discovery R92TC-3 RIPA Buffer ThermoFisher Scientific 89901 RPMI 1640 Gibco 61870-036 RPMI 1640 Sigma R0883 RPMI 1640 without phenol red Gibco 32404014 Sodium pyruvate Sigma S8636 Sodium pyruvate Gibco 11360070 Sodium selenite Sigma S5261 Stain buffer (with FBS or BSA) BD Pharmingen 554657 or 554656 Surfactant Meso Scale Discovery R92TC-2 Sytox Blue ThermoFisher Scientific S11348 Sytox Green Dead Cell Stain ThermoFisher Scientific S34860 TBST (TBS with 0.1% Tween20) Bio-Rad 170-6531 Total protein assay module ProteinSimple DM-TP-01 T-PER buffer Pierce 78510 Transfer Buffer Novex NP0006-1 V-PLEX and V-PLEX Plus Proinflammatory Panel 1 (human) Kit Meso Scale Discovery K15049D Wash buffer (1XDPBS, 0.05% Tween-20) Janssen R&D N/A XCell II Blot Module ThermoFisher Scientific EI9051 Xylene VWR 28975-325 Critical commercial assays Anti-HQ-HRP detection kit Roche 701793601 BCA Protein Assay Kit ThermoFisher Scientific 23227 Bond Polymer Refine Detection Kit Leica Biosystems DS9800 DISCOVERY ChromoMap DAB kit Roche 760-159 MACS Human Tumor/Xenograft Dissociation kit Miltenyi Biotec 130-095-929 MACS Mouse Tumor Dissociation kit Miltenyi Biotec 130-096-730 Mouse cell Depletion Kit Miltenyi Biotec 130-104-694 OmniMap DAB anti-Rb Detection Kit Roche 760-149 sHLA-G ELISA kit ExBio RD194070100R T Cell Activation / Expansion Kit, human Miltenyi Biotec 130-091-441 Trypan blue 0.4% Gibco 15250 TrypLE Gibco 12563-029 TrypLE Express Gibco 12605036 TrypLE select Invitrogen 12563-011 TrypLE TM Select Enzyme (13), no phenol red Thermo Fisher Scientific 12563011 UltraPure LMP agarose Invitrogen 16520-050 IL-2 Miltenyi Biotec 130-097-743 Insulin Gibco 12585-014 (Continued on next page) e3 iScience 28, 111876, March 21, 2025 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant human IFN-y R&D Systems 285-IF-100 Recombinant human IL-6 R&D Systems 206-IL-200 Recombinant human TNF-a R&D Systems 210-TA-100 Transferrin Sigma T8158 Biotinylated sHLA-G (MHGW8.ECO.PP.DB.002) Janssen R&D NA 0.5 mL Polypropylene assay blocks Bio-one 786261 1.2 mL Deepwell 96-Well plate Assay, polypropylene ThermoFisher Scientific, Abgene AB1127 10 mL Syringe BD Biosciences 301304 15 mL Conical tube Falcon 352097 6-well Clear flat bottom cell culture plate Corning 353046 70-mm Strainer Falcon 352350 96-Well Polypropylene plate Greiner 650201 or 786261 96-Well V bottom plate Corning 3894 96-Well Microplate, Polypropylene, U – Bottom, Chimney Style, Clear, No Lid Greiner Bio-One 650261 96-Well E-Plates ACEA Biosciences/Agilent 5232368001 AbgeneTM 96-Well Polypropylene DeepWell Storage Plates ThermoFisher Scientific AB1127 Albumin standard Pierce 23209 BD Microtainer tube (serum separator) VWR VT365967 Breath-easy membrane Sigma Z380059 Cell Strainer, 70 mm, white Falcon 352350 Deep well storage plates, 96-well, 2.2 ml, Mark II VWR, Abgene 732-4910 Falcon 50 ml conical tubes ThermoFisher Scientific 12716688 Falcon 96-well U-bottom plate Corning 353077 Falcon Centrifuge Tubes, Polypropylene, Sterile, 50 mL Conical Tube Corning 352070 Flat bottom tissue culture plate 96-well Corning 3598 GentleMACS C-Tubes Miltenyi 130-093-237 iBlot Gel Transfer Stacks, PVDF regular Invitrogen IB401001 Immun-Blot PVDF membrane Bio-Rad 162-0177/ 162-0174 Liquid coverslip Roche 650-10 Low attachment 96-well U-bottom plate Costar 7007 Lysing Matrix D tube MP Biomedicals 6913-100 MSD 10-plex plates Meso Scale Discovery K15235N MSD 96-Well Streptavidin Gold Plate Meso Scale Discovery L15SA-1 MSD Small Spot Plate Meso Scale Discovery L45SA-1 Nunc seal Nunc 236366 Sterile plate covers/ Lid Universal Clear Sterile PS ThermoFisher Scientific 250002 Sterile U-bottom 96-wells plates for flow Falcon 351177 TC 5-layer flask, 875 mm2 Falcon 353144 V-bottom 96-well plate Nunc NUNC249952 V-bottom polypropylene 96 well plates Greiner 65261 VWR Micro slides, Superfrost Plus VWR 48311-703 Experimental models: Cell lines .. RERF-LD-Ad1 Radiation Effects Research Foundation JCRB1020 BICR6 Sigma-Aldrich 5070501 (Continued on next page) iScience 28, 111876, March 21, 2025 e4



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